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CDNA Synthesis Kit

Kit - bei Amazon.d

  1. Niedrige Preise, Riesen-Auswahl. Kostenlose Lieferung möglic
  2. Laborbedarf und Labortechnik in top Qualität für flexible Einsatzmöglichkeiten! Ihr kompetenter Partner für PCR Kits. Hier direkt online informieren
  3. imal setup and reaction time. Key Features and Benefit
  4. The SMARTer PCR cDNA Synthesis Kit includes the same components as the original SMART PCR cDNA Synthesis Kit plus an improved SMARTer II A oligo and SMARTScribe Reverse Transcriptase, which provide higher specificity and increased yield. Notice to purchaser . Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in.

PCR Kits - LTF Labortechnik Gmb

  1. Biozym cDNA Synthesis Kit. Zwei Tube Mix - ergonomisch, inkl. Primer. Effiziente Synthese für lineare Repräsentation aller Transkripte; Ideal für qPCR; Ergonomisches Zwei-Tube System, inkl. effektivem RNase-Inhibitor; Random Hexamer- und oligo (dT)-Primer fertig im Mix; Produkte ; Biozym cDNA Synthesis Kit 25 rxn à 20 µl . Art.-Nr.: 331475S. € 75,00. merken : Biozym cDNA Synthesis Kit.
  2. Führen Sie eine effiziente Synthese von cDNA aus RNA-Templates mit dem kompletten System im Thermo Scientific™ Fermentas Erststrang cDNA-Synthese-Kit durch. Das Kit verwendet die rekombinante M-MuLV-Reverse Transkriptase, die eine geringere RNase H-Aktivität aufweist als die AMV-Reverse Transkriptase
  3. g from internal sites of the poly (A) tail
  4. The High Capacity cDNA Reverse Transcription Kit delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 µg total RNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives
  5. cDNA Synthesis System; Instructions For Use Optimized one-tube procedure for the synthesis of double-stranded cDNA up to 3 kb from total RNA or mRNA. The cDNA Synthesis System uses an optimized one-tube procedure, based on the method of Gubler and Hoffmann. Sample: 1 - 20 μg total RNA, 0.3 - 2 μg mRN
  6. ation and cDNA synthesis into one-tube procedure

iScript™ cDNA Synthesis Kit Life Science Research Bio-Ra

  1. imum of one year from date of purchase. Thaw, mix, and.
  2. g reverse transcription qPCR and cDNA synthesis for qPCR, you need reagents that deliver high specificity and sensitivity and process safety. Our RT-qPCR kits have a unique combination of in-process controls - including a built-in visual indicator - to remove variables and increase the accuracy, specificity and sensitivity of your analyses
  3. The PrimeScript 1st strand cDNA Synthesis Kit contains all of the reagents necessary for synthesis of full-length first-strand cDNA from total or poly A + RNA, including both oligo (dT) and random 6-mer primers
  4. The SensiFAST™ cDNA Synthesis Kit provides a rapid and sensitive method for first-strand cDNA synthesis, which displays excellent linearity across a wide range of starting material. This gives the same relative representation in cDNA templates, regardless of gene abundance, making it excellent for use in qPCR studies

iScript Select cDNA Synthesis Kit is designed for applications requiring high-fidelity, such as cloning and NGS. This kit introduces fewer base pair errors than other commonly used reverse transcription kits qScript cDNA Synthesis Kit was used for first-strand cDNA synthesis using log-fold serial dilutions of HeLa cell total RNA from 1 μg to 1 pg. Six replicate cDNA reactions were performed for each input quantity of RNA. 1/10th of each first-strand reaction was used for qPCR of the b-actin gene using PerfeCTa SYBR Green SuperMix. < > Consistent Efficiency . Consistent Efficiency. Consistent cDNA. When performing reverse transcription and cDNA synthesis, your experiments' success can be impacted by a number of critical factors including the efficiency of the reverse transcriptase and speed of the reverse transcription process, which is vital for optimal cDNA synthesis. Our cDNA synthesis kits overcome the challenges of cDNA synthesis and deliver high yields of cDNA thanks to high.

This kit enables the synthesis of ≥ 14 kb cDNA. ReverTra Ace-α-™ is an efficient and convenient kit to synthesize high quality cDNA. This kit contains the highly efficient reverse transcriptase ReverTra Ace™, as well as other components optimized for the synthesis of long cDNAs suitable for RT-PCR High quality cDNA synthesis is essential for downstream real-time PCR analysis and successful expression studies. qPCRBIO cDNA Synthesis Kit is an easy-to-use 2 tube system specifically developed to generate cDNA for use in real-time PCR biotechrabbit™ cDNA Synthesis Kit provides superior components that ensure efficient first-strand cDNA synthesis from mRNA or total RNA templates. biotechrabbit RevertUP™ II Reverse Transcriptase enables highly efficient reverse transcription with increased thermostability. biotechrabbit RNase Inhibitor is a potent non-competitive inhibitor of RNases. The combination of highly efficient. SCRIPT cDNA Synthesis Kit contains all reagents required for first strand cDNA synthesis in one box combining simple handling with high flexibility. The premium quality Reverse Transcriptase, ultrapure dNTPs and an optimized reaction buffer ensure superior results with highest reproducibility

ProtoScript® First Strand cDNA Synthesis Kit features two optimized mixes, M-MuLV Enzyme Mix and M-MuLV Reaction Mix. M-MuLV Enzyme Mix combines M-MuLV Reverse Transcriptase and Murine RNase Inhibitor, while M-MuLV Reaction Mix contains dNTPs and an optimized buffer. The kit also contains two optimized primers for reverse transcription and nuclease-free water. An anchored oligo-dT primer [d(T. The qScriber™ cDNA Synthesis Kit is a highly efficient and simple-to-use system for cDNA synthesis eliminating the need for tedious reaction optimization. The qScriber™ Enzyme Blend ensures high sensitivity detection from low copy number targets First-Strand cDNA Synthesis Kit For the generation of full-length first-strand cDNA from an RNA template using a variety of primers Product Booklet Code: 27-9261-01. 2 Page finder 1. Legal 3 2. Handling 4 2.1. Safety warnings and precautions 4 2.2. Storage 4 2.3. Expiry 4 3. Components 5 4. Other materials required 6 5. Description 7 6. Critical parameters 8 7. Protocol 9 7.1. Introduction 9 7. Das Maxima First Strand cDNA Synthesis Kit für die RT-qPCR ermöglicht eine reproduzierbare cDNA-Synthese aus unterschiedlichen Mengen von Gesamt-RNA (1 pg bis 5 μg) bei erhöhten Temperaturen (42 bis 65 °C). Die Synthesereaktion kann innerhalb von 15 bis 30 Minuten abgeschlossen werden. Komponenten des Maxima First Strand cDNA Synthesis Kit für die RT-qPCR sind bereits vorgemischt. Dies.

The synthesis of DNA from an RNA template, via reverse transcription, produces complementary DNA (cDNA). Reverse transcriptases (RTs) use an RNA template and a short primer complementary to the 3' end of the RNA to direct the synthesis of the first strand cDNA, which can be used directly as a template for the Polymerase Chain Reaction (PCR) The Reliance Select cDNA Synthesis Kit yields highly consistent results, regardless of whether RNA is intact or degraded (using heat treatment). Using housekeeping genes, we demonstrate a lower ΔCq value between reverse transcription kits with intact and degraded samples. The Reliance Select Kit delivers earlier Cqs compared to the SuperScript IV System when tested with artificially degraded. Das Maxima First Strand cDNA Synthesis Kit für die RT-qPCR ermöglicht eine reproduzierbare cDNA-Synthese aus unterschiedlichen Mengen von Gesamt-RNA (1 pg bis 5 μg) bei erhöhten Temperaturen (42 bis 65 °C). Die Synthesereaktion kann innerhalb von 15 bis 30 Minuten abgeschlossen werden NZY First-Strand cDNA Synthesis Kit is formulated to provide high yields of full-length cDNA products and to increase sensitivity in RT-qPCR. Starting material can range from 10 pg up to 5 µg of total RNA. The kit includes a combination of random hexamers and oligo (dT) 18 primers in order to increase sensitivity

cDNA synthesis kits - Takara Bi

cDNA synthesis kits provide a fast and reliable means of producing cDNA from RNA samples. These convenient ready-to-use kits come with the components necessary for reverse transcription, including the reverse transcriptase (RT), dNTPs, reaction buffer, nuclease-free water, and RNase inhibitors The kit contains all necessary components including a highly sensitive and thermostable recombinant reverse transcriptase which provides enhanced cDNA synthesis efficiency, high fidelity, and a wide range of activity for varying amounts of RNA templates (0.1 ng to 2 µg). Due to its high thermal stability, low RNase activity and resistance to inhibition by rRNA and tRNA, the recombinant RT. First-Strand cDNA Synthesis Kit For synthesis of single-stranded cDNA prior to amplification by PCR Preassembled bulk reaction mixes save time and minimize potential for pipetting errors Allows users to choose primers Includes a choice of two primers: pd (N) 6 random hexamers and NotI- (dT)1 Mint-2 cDNA synthesis kit is based on a novel technology (patent pending) utilizing the specific features of MMLV-based reverse transcriptase (RT). First strand cDNA synthesis starts from the 3´-end CDS adapter containing an oligo (dT) sequence that anneals to poly (A) stretches of RNA

The µMACS and MultiMACS cDNA Synthesis Kits facilitate fast and efficient mRNA isolation, cDNA Synthesis, and cDNA purification, all in one column, without prior preparation of total RNA. Pure mRNA is directly isolated from cells, tissue, or blood by magnetic labeling with µMACS Oligo(dT) MicroBeads. Due to the fast reaction kinetics the small (50 nm) µMACS Oligo(dT) MicroBeads instantly. ProtoScript ® II First Strand cDNA Synthesis Kit: E6560S: 30 reactions: 166 € E6560L: 150 reactions: 664 € AMV Reverse Transcriptase: M0277S: 200 units: 75 € M0277L: 1.000 units: 300 € M-MuLV Reverse Transcriptase: M0253L: 50.000 units: 284 € M0253S: 10.000 units: 71 € Murine RNase Inhibitor: M0314L: 15.000 units: 296 € M0314S: 3.000 units: 74 € Passend dazu Our cDNA synthesis kits overcome the challenges of cDNA synthesis and deliver high yields of cDNA thanks to high-affinity enzymes. Plus, you don't need to perform an additional RNase H digestion step Unlike other cDNA synthesis kits which can only prepare one or two classes of RNAs for qPCR, the RNA-Quant Kit enables qPCR measurement of many classes of RNA from a single cDNA synthesis reaction. The kit includes three endogenous RNA reference qPCR assays as for human and mouse samples—miR-16, Y RNA lncRNA, and GAPDH mRNA. An all-in-one cDNA synthesis kit. Tail and tag all RNAs for cDNA.

TaqMan Advanced miRNA cDNA Synthesis Kit - Thermo Fisher

Biozym cDNA Synthesis Kit

  1. First Strand cDNA Synthesis Kit ProtoScript II First Strand cDNA Synthesis Kit AMV First Strand cDNA Synthesis Kit NEBNext RNA First Strand Synthesis Module FastGene 55-Scriptase cDNA Syntehsis Kit qPCRBIO cDNA Synthesis Kit GoScript Reverse Transcription System Reverse Transcription System QuantiNova Reverse Transcription Kit OmniScript RT Kit (50 / 200
  2. - Optimized for various downstream applications, including next generation sequencing - High content of full length transcripts Mint-2 cDNA synthesis kit is designed to synthesize full-length-enriched double stranded (ds) cDNA from total or poly (A)+ RNA
  3. The AzuraFlex™ cDNA Synthesis Kit is a complete and flexible system for efficient synthesis of first strand cDNA from Total RNA or mRNA templates. The MMLV-derived reverse transcriptase (High Capacity RTase) is modified for improved sensitivity with challenging templates, and greater thermostability. The enzyme, which is capable of synthesizing cDNA up to 9Kb is blended with RNase inhibitor.

Norgen's microScript microRNA cDNA Synthesis Kit is an all-in-one, ready-to-use product for the reverse transcription of microRNA from either Total RNA preparations or enriched microRNA preparations. The kit contains the 2x Reaction Mix and the microScript microRNA Enzyme Mix qScript cDNA SuperMix is a sensitive and easy-to-use 1-tube reagent for first-strand cDNA synthesis that combines a highly-modified RNAse H+ mutant of M-MLV together with ribonuclease inhibitor protein (RIP) in a rigorously optimized formulation for real-time qPCR applications Hiscript III 1 st Strand cDNA Synthesis Kit (+ gDNA wiper) is an upgraded version of Hiscript II 1st Strand cDNA Synthesis Kit (+ gDNA wiper) containing a new generation of reverse transcriptase Hiscript III Reverse Transcriptase and Buffer optimized for reverse transcription. This product improves the efficiency of first strand cDNA synthesis 2 AffinityScript Multiple Temperature cDNA Synthesis Kit INTRODUCTION The AffinityScript Multiple Temperature cDNA Synthesis Kit is ideal for the production of high yields of cDNA from a wide range of total RNA or mRNA amounts. The kit includes our AffinityScript Multiple Temperatur

First Strand cDNA-Synthese Kit Direkte PCR-Reagenzien und

  1. g - oligo (dT), random primers or gene-specific primers Robust cDNA synthesis from any RNA template High reproducibility and excellent yiel
  2. imum of one year after ship date. Nuclease-free water can be stored at room temperature
  3. The kit uses the Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus (M-MLV RT (H-)) which is an RNA-dependent DNA polymerase that is used in cDNA synthesis with long RNA templates. The lack of RNase H activity is important in this application in that RNase H activity will start to degrade template during long incubation times which are required for producing long cDNAs. RNase.

Supporting Data. Get robust cDNA synthesis with EVeryRNA. To demonstrate the excellent RNA yields and robust cDNA synthesis obtained with EVeryRNA, we isolated EVs from 250 µL of serum using SmartSEC Single, spiked in 0.1 pmol of Cel-miR-39, and used both the EVeryRNA EV Purification System and a phenol-based kit to isolate RNA. The isolated RNA was reverse transcribed using the EVeryRNA cDNA. The TonkBio™ First Chain cDNA Synthesis Kit for RT-PCR is optimized to efficiently synthesize first chain cDNA from mRNA or total RNA templates. The kit uses M-MLV reverse transcriptase with lower RNase H activity, which avoids degradation of RNA when RNA-DNA hybrid forms in the progress of cDNA synthesis. It is suitable for synthesis of cDNA up to 9 kb. The cDNA products could be used.

Transcriptor First Strand cDNA Synthesis Kit

Protocol for cDNA synthesis and qRT-PCR cDNA Synthesis Superscript III 1st Strand Synthesis Kit (Invitrogen, Cat #18080-051) We typically use 1μg of Total RNA per 20μl cDNA reaction, but you may be able to use less. Initial Primer/RNA Mix: 0.5 μl random hexamer primers 0.5 μl oligo(dT) primers 1μl dNTP mix 8ul of RNA (1μg) + DEPC H 20 Heat to 95°C, 5 min Buffer/Enzyme mix (assemble in. MG cDNA Synthesis Kit; MG cDNA Synthesis Kit II; MG 2X qPCR MasterMix (SYBR Green) MG 2X qPCR MasterMix (TaqMan) MG 2X qPCR MasterMix (SYBR Green) with ROX; MG 2X qPCR MasterMix (TaqMan) with ROX; MG 2X One Step RT-PCR MasterMix (SYBR Green) MG 2X One Step RT-PCR MasterMix (TaqMan) MG 2X One Step RT-PCR MasterMix with Loading Dy cDNA Synthesis kit for miRNA—50 reactions. *Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product EasyScript cDNA Synthesis Kit is a complete system for the efficient synthesis of first strand cDNA from RNA templates. The recombinant RNasin Ribonuclease Inhibitor, supplied with the kit effectively protects RNA template from degradation. The kit is also supplied with both oligo(dT) and random primers. The oligo(dT) anneals selectively on the poly(A) tail of mRNA. Random primers do not.

High-Capacity cDNA Reverse Transcription Kit

cDNA Synthesis Kit Sigma-Aldric

Either the Transcriptor First Strand cDNA Synthesis Kit (blue curves) or another suppliers's Reverse Transcriptase (red curves) was used to perform reverse transcription on 100 ng to 10 pg of total RNA from K-562 cells. LightCycler FastStart DNA MasterPLUS SYBR Green I‡,§ was then used with primers for G6PDH to quantify gene-expression levels NZY First-Strand cDNA Synthesis Kit quantity. Add to cart. NZY First-Strand cDNA Synthesis Kit, separate oligos (MB170 ) € 175,00 - € 775,00. Quantity: Clear: NZY First-Strand cDNA Synthesis Kit, separate oligos quantity. Add to cart. NZY M-MuLV First-Strand cDNA Synthesis Kit (MB172 ) € 135,00 - € 595,00. Quantity: Clear: NZY M-MuLV First-Strand cDNA Synthesis Kit quantity. Add to. UltraScript 2.0 cDNA Synthesis Kit Separate Oligos provides anchored oligo(dT) and random hexamers in separate tubes, for user optimisation depending on the type of analysis needed. Applications. cDNA synthesis for PCR analysis, cloning, cDNA library preparation and Next Generation Sequencing; Viral RNA targets ; miRNA targets; Efficient synthesis from total RNA or poly(A)+ RNA; Specifications. Flexible cDNA synthesis kit with fast and robust FIREScript ® Reverse Transcriptase. Genetically modified MMLV-based reverse transcriptase with increased thermostability and improved performance at elevated temperatures. high specificity and yield; wide reaction temperature from 37°C to 60°C; fast 15 min reaction tim Accuris now offers two different cDNA synthesis kits, to meet a range of requirements. Our original cDNA Synthesis Kit comes as a convenient 2-tube format for easy reaction set up and is ideal for 4pg to 0.5µg of input RNA.. The new First Strand cDNA Synthesis Flex Kit includes four components: Reverse Transcriptase, Buffer, oligo (dT) primers, and random hexamer primers

cDNA Synthesis Kit contains all the necessary components to generate cDNA from an RNA template. The generated cDNA is suitable for PCR with gene-specific primers or for other downstream applications. The kit contains MMLV Reverse Transcriptase and is suitable for first strand cDNA synthesis, cDNA The BioLink™ cDNA Synthesis Kit provides a rapid and sensitive method for generating high-quality, qPCR-ready first-strand cDNA for use in downstream real-time PCR. It is ideal for 4pg to 0.5µg of input RNA and includes two tubes for easy reaction set up. One tube contains our exceptionally stable reverse transcriptase and a powerful RNase inhibitor. The second tube contains a 5X reaction. Scientific™ DyNAmo™ cDNA Synthesis Kit is intended for cDNA synthesis for two-step quantitative reverse transcription-PCR (qRT-PCR) applications, where amplicons are usually around 100 bp in length. This kit can be used in conjunction with DyNAmo qPCR Kits or with any other qPCR kit suitable for the application. The DyNAmo cDNA Synthesis Kit includes all the necessary reagents for.

Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with

The original cDNA Synthesis Kit comes as a convenient 2-tube format for easy reaction set up and is best for 4pg to 0.5µg of input RNA. One tube includes the exceptionally stable Reverse Transcriptase combined with a potent RNAse inhibitor, and the other tube contains a 5X reaction buffer with an optimal mixture of anchored oligo (dT) primers and random hexamers to produce a non-biased. SensiFAST cDNA Synthesis Kit contains a highly-pure reverse transcriptase and optimized TransAmp™ buffer system, which includes a unique blend of random hexamers and anchored oligo (dT) primers to deliver the highest quality qPCR ready cDNA. This makes the SensiFAST cDNA Synthesis Kit ideal for working with limited sample volumes, such as laser-micro dissected samples and tissue biopsies. AffinityScript QPCR cDNA synthesis kit provides a streamlined master mix format and fast protocol, with a 15-minute cDNA synthesis step and an overall protocol time of just 25 minutes. The kit is formatted for high-yield production of cDNA up to 12 kb and each reaction accommodates a range of RNA amounts from fg to µg. Advantages of the AffinityScript QPCR cDNA Synthesis Kit • Robust cDNA.

Introducing Reliance Select cDNA Synthesis Kit. Don't let difficult samples stand between you and your data. The Reliance Select cDNA Synthesis Kit was desig.. AMPIGENE® cDNA Synthesis Kit for efficient and sensitive cDNA synthesis, for downstream qPCR.Die umfangreiche Auswahl an Reagenzien eignet sich für die Anwendung an Nukleinsäuren in Transfektions- und Transformationsverfahren sowie bei der Klonierung, Sequenzierung, Aufreinigung und Extraktion. Nutzen Sie diese Reagenzien zum Isolieren und Aufreinigen von Nukleinsäuren aus biologischem.

iScript™ Advanced cDNA Synthesis Kit for RT-qPCR | Life

The RevertAid™ First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb. The kit uses RevertAid™ Reverse Transcriptase which has lower RNase H activity compared to AMV reverse transcriptase. The recombinant RiboLock™ RNase Inhibitor, supplied with the kit, effectively. The AzuraQuant™ cDNA Synthesis Kit provides a rapid and sensitive method for generating high quality, qPCR-ready first-strand cDNA for use in downstream real-time PCR. The AzuraQuant™ cDNA Synthesis Kit exhibits highly reproducible, unbiased synthesis and representation of 5' and 3' mRNA ends. The Kit is supplied in a convenient, two tube format comprised of a 5x Buffer and 20x HighTherm. cDNA-Synthese Kits Kitsystem mit allen Komponenten für die Erststrang-cDNA-Synthese Inklusive genetisch modifizierter M-MuLV Reverser Transkriptase und RNase Inhibitor Zwei Varianten mit M-MuLV H Plus und H Minus mit und ohne RNase H Aktivität selektiv an die 3'-Enden polyadenylierter RNA bei der cDNA-Synthse mit mRNA. Für die Synthese von cDNA einer ganz bestimmten Sequenz können in. cDNA synthesis core kit. Filters. One Step kit converter; PROBE One-Step; cDNA synthesis core kit; 1 - 1 of 1. Reverse Transcriptase Core Kit Quick view 1 - 1 of 1. Products ; Endpoint PCR; Quantitative PCR ; Gene purification & Extraction; Electrophoresis System; Antibodies; Peptides; Recombinant proteins. The kit contains all necessary components including a highly sensitive and thermostable recombinant reverse transcriptase which provides enhanced cDNA synthesis efficiency, high fidelity, and a wide range of activity for varying amounts of RNA templates (0.1 ng to 2 µg). Due to its high thermal stability, low RNase activity, and resistance to inhibition by rRNA and tRNA, the recombinant RT.

Using the SuperScript III CellsDirect cDNA Synthesis System, the cells are lysed and the cDNA is generated from the lysate in a single tube with minimal handling and no sample loss. DNase I is added to eliminate genomic DNA prior to first-strand synthesis. This kit has been optimized for small cell samples, ranging from 10,000 cells down to a single cell (as measured by serial dilution). The. The iScript kit gave me no visible bands and the SuperScript kit gave me an incredibly faint band. I have tried using 200 ng, 100 ng, and 50 ng of RNA to make the cDNA and all amounts give me the. RT-PCR kits and standalone reverse transcriptase enzymes for reverse transcription of full-length cDNA from your experimental sample. Includes GoScript™ Reverse Transcriptase, M-MLV and AMV reverse transcriptases. GoScript uses M-MLV reverse transcriptase and state-of-the-art buffer to drive robust, reliable cDNA synthesis of a full range of rare and abundant transcripts Reliable cDNA Synthesis for Sensitive Detection of Expression. Available as a standalone enzyme, a complete reverse transcription kit or as a master mix with Oligo(dT) or Random Primers; Sensitive transcription of both high-copy and low-copy messages; Transcribes short and long transcripts; processes through secondary structur

AMPIGENE ® cDNA Synthesis Kit uses the latest developments in reverse transcriptase (RTase) technology and buffer chemistry for efficient and accurate cDNA synthesis. The modified MMLV RTase is blended with RNase inhibitor to prevent degradation of RNA by contaminating RNase. The RTase is thermostable and extremely active. An optimized buffer system contains oligo(dT), random hexamers. qScript™ cDNA Synthesis Kit contains qScript™ Reverse Transcriptase, a mixture of a MMLV reverse transcriptase derivative and ribonuclease inhibitor protein, optimised for sensitive and reliable cDNA synthesis over a broad dynamic range of input RNA. cDNA synthesis reagents are provided in a variety of SuperMix and kit formats to suit specific applications reverse transcription cDNA synthesis . Quelle E.coli . Sicherheits Hinweise / Safety Klassifizierungen / Classification eclass-Nr: 34-16-04-90. Dokumente. Dokumente - Protokolle - Downloads mehr. Zurück . Dokumente - Protokolle - Downloads. Hier finden Sie Informationen und weiterführende Literatur zu One-Step cDNA RT-PCR Kit (2X). Für weitere Dokumente (Zertifikate mit weiteren Lotnummern. cDNA Synthesis Kit Cat No: YT4500 for research use only Size: 50 tests < store at -20ᴼC > Contents: M-MLV (10,000 U) 200u/ µl 50 µl Important Note : 5X first-strand Buffer 200 µl Primers are lyophilized. Oligo(dT)18 primer * Please add DEPC water used for Con.50 µM Random hexamer primer ** * Oligo(dT)18 primer : 60.96 µl RNasein (40u/µl) 25 µl ** Random Hexamer : 184.48 µl dNTP 10mM. PRODUCTS | cDNA synthesis kits | ReverTra Ace™ qPCR RT Master Mix with gDNA Remover | The category leader, continuing to create new value that contributes to society in the environment, healthcare, and high-function product fields

SensiFAST cDNA Synthesis Kit - Biolin

rtStar™ cDNA Synthesis products offer four types of kits to meet the needs of studying different RNAs. They are suitable for reverse transcribing total RNAs, tRNAs, miRNAs and other small RNAs, respectively The qScript™ microRNA cDNA synthesis kit has been designed to convert miRNAs into cDNA starting from total RNA or RNA preparations pre-enriched for miRNAs. miRNAs are not polyadenylated in nature. With the qScript™ microRNA cDNA synthesis kit miRNAs are polyadenylated in a poly(A) polymerase reaction. qScript™ reverse transcriptase and other necessary reagents for cDNA synthesis are.

Strand-switching protocols for preparation of 1D and 2D cDNA libraries. The Ligation Sequencing Kit contains a protocol for 1D cDNA sequencing. Following first-strand synthesis, reverse transcriptases tend to add 1-3 non-templated Cs to the 3' end of the cDNA strand, and by including an additional primer in the reaction which anneals to the non- templated Cs, we can make the RT enzym The First Strand cDNA Synthesis Kit is designed for the preparation of high quality first strand cDNA from cellular RNA templates. The kit contains MMLV Reverse Transcriptase for superior yields of full-length cDNA. Both oligo(dT) and random hexamer primers are included for a choice of general priming strategies and as alternatives to user-supplied specific primers. A small volume (1-2 µl) of. miRNA 1st Strand cDNA Synthesis Kit (by stem-loop),RNA/miRNA,RNA Regent/RT-PCR,Products center,Vazyme Biotech Co.,Ltd The full-length cDNA synthesis takes around 5 hours in total, including just 60 minutes of hands-on time. Endpoint PCR for amplification of TeloPrime cDNA using the V2 kits takes between 5 - 8 hours (or depending on the number of PCR cycles) with only 25 minutes are hands-on time. The qPCR, which is strongly recommended, is performed prior to.

SMARTer PCR cDNA Synthesis Kit Clontech (634925 or 634926) KAPA HiFi PCR Kit Kapa Biosystems (KK2101 or KK2102) PrimeSTAR GXL DNA Polymerase Clontech (R050A or R050B) Additional 5' PCR Primer IIA Any Oligo Synthesis Vendor 1.2% FlashGel system or 0.80% Agarose Gels Lonza Any MLS FlashGel DNA Marker (100 bp - 4 kb) Lonza Nanodrop Thermo Scientific DNA 7500 Bioanalyzer Kit DNA High Sensitivity. SOLIScript ® RT cDNA synthesis KIT. A flexible and highly specific cDNA synthesis kit contains all the reagents you need for first-strand cDNA synthesis. Individual components enable thorough assay optimization. Note! The kit includes oligo dT primers, SOLIScript ® is not recommended with random primers. SOLIScript ® RT cDNA synthesis MIX. All-inclusive and the most convenient product. AMPIGENE ® 1-Step RT-PCR Kit uses the latest developments in reverse transcriptase (RTase) technology and buffer chemistry for efficient cDNA synthesis and PCR in a single tube. The modified MMLV RTase is blended with RNase inhibitor to prevent degradation of RNA by contaminating RNase. The RTase is thermostable and extremely active

Reliance Select cDNA Synthesis Kit contains a newly engineered reverse transcriptase designed to perform well with challenging samples and targets. This kit has the capability of processing samples with difficult inhibitors and degraded RNA (e.g., FFPE samples) as well as targets containing secondary structures and/or high GC content. Features and Benefits. Novel engineered reverse. SensiFAST cDNA Synthesis Kit displays excellent linearity across a wide range of starting material, revealing the same relative representation in your cDNA template, regardless of gene abundance. Furthermore, SensiFAST cDNA Synthesis Kit will produce reliable, high performance results, in both real-time and conventional RT-PCR. A reverse transcriptase and new TransAmp ™ buffer system. First-Strand cDNA Synthesis Kit是基于Reverse Transcriptase(货号:K1071)从Total RNA或Poly(A)+RNA合成第一链cDNA的试剂盒。Reverse Transcriptase是在M-MLV (RNase H-) Reverse Transcriptase基础上经过基因工程改造后获得的全新逆转录酶,相较而言Reverse Transcriptase降低了RNase H活性且大幅度提高了热稳定性 MG cDNA Synthesis Kit contains MMLV Reverse Transcriptase and reagents for cDNA synthesis. It consists of RNase Inhibitor, reaction buffer, dNTPs and two optimized primers. An oligo-dT(15) primer anneals to the beginning of the poly A tail of mRNAs. The optimized Random Primer provides random and consistent priming covering the entire RNA template including both mRNAs and non-polyadenylated. cDNA Synthesis kit for miRNA—10 reactions. The use of this Others has been cited in the following citations:; Downregulation of VDR and miR-126-3p expression contributes to increased endothelial inflammatory response in preeclampsia,Xu, J;Gu, Y;Lewis, DF;Cooper, DB;McCathran, CE;Wang, Y;, Am. J. Reprod. Immunol. 2019,PubMed ID 31323164 [miRNA cDNA synthesis kit

The Xpert cDNA Synthesis Kit provides an efficient and fast method for the synthesis of high quality first strand cDNA from purified poly(A)+ mRNA or total RNA templates. The Xpert cDNA Synthesis core kit includes Xpert Reverse Transcriptase (RNase H-), a RNA-dependent DNA polymerase suitable for cDNA synthesis from long RNA templates. Xpert RTase has been optimized to perform under high. Viva cDNA Synthesis Kit is specially designed to provide reliable synthesis of full-length cDNA. M-MuLV RNase H- synthesizes complementary DNA strand initiating from a specific primer, oligo d(T) or random hexamer. The absence of RNase H enhances the synthesis of long cDNA as the RNA strand does not degrade in DNA-RNA hybrid during first strand cDNA synthesis. This cDNA synthesis kit is.

VitaScript™ first strand cDNA Synthesis Kit contains: COMPONENT VOLUME DESCRIPTION VitaScript™ Enzyme Mix 50 µl contains VitaScript™ Reverse Transcriptase and RNase inhibitor blend 5X VS Reaction Buffer 250 µl contains dNTPs, MgCl 2, random primer and oligo-dTs in optimized buffer environment Nuclease-free water 1 ml GENERAL CONSIDERATIONS • High purity template RNA is essential for. The OrientExpress™ cDNA Synthesis and Cloning Systems are complete sets of reagents designed for rapid, efficient construction of cDNA libraries having inserts in a defined orientation. Six systems are available based on the following vectors: a bacteriophage lambda insertion vector, λSCREEN-1, and two bacteriophage T7 vectors, T7Select1-1 and T7Select10-3. Each vector is available as a kit. Description: The First Strand cDNA Plus Synthesis kit contains all reagents for first strand cDNA synthesis and offers a simple handling with high flexibility. A genetically engineered version of M-MLV Reverse Transcriptase with no RNase H activity is a RNA-directed DNA enzyme that synthesizes a complementary DNA strand initiating from a primer using single-stranded RNA or DNA as template. BiomarkerScript II 1st Strand cDNA Synthesis Kit enables synthesis of cDNA upto 10 kb in length, which is suitable for gene cloning. Applications First strand cDNA synthesis. Reverse transcription products for qPCR. Reverse transcription products for gene cloning. cDNA library construction . About us. Biomarker develops main businesses such as technology services, medical genetic testing and.

The miRNA 1st-strand cDNA synthesis kit can accommodate a range of total RNA input amounts from 30 ng to 1 μg. The optimal quantity of RNA template depends on the RNA purity and the expression level of the particular miRNA of interest. Use of Manganese Chloride Manganese chloride is provided in the miRNA 1st-strand cDNA synthesis kit as an optional reagent in the polyadenylation reaction. First-Strand cDNA Synthesis Kit is an Efficient & Sensitive System for First strand cDNA Synthesis from RNA templates. It includes s all the necessary components to synthesize first-strand cDNA, except the template RNA (total RNA or mRNA). First-Strand cDNA Synthesis Kit features two optimized mixes, RT Enzyme Mix and RT Reaction Mix. RT Enzyme Mix combines Reverse Transcriptase and RNase. RNA Synthesis Kits for mRNA Synthesis. Selected References [1] Karikó et al.(2005) Suppression of RNA Recognition by Toll-like Receptors: The Impact of Nucleoside Modification and the Evolutionary Origin of RNA.Immunity23:165. [2] Karikó et al.(2008) Incorporation of Pseudouridine into mRNA Yields Superior Nonimmunogenic Vector With Increased Translational Capacity and Biological Stability Choose your priming methods with crb's cDNA Synthesis Kit. crb's cDNA Synthesis kit provides a comprehensive set of reagents necessary to generate high quality cDNA and offers the most flexibility in respect to priming methods and reaction optimization. Both random primers and oligo(dT) are included for a choice of general priming strategies and as alternatives to gene-specific primers.

Evrogen: Mint cDNA synthesis tecnology

The iScript cDNA synthesis kit is a sensitive and easy-to-use first-strand cDNA synthesis kit for gene expression analysis using real-time qPCR. This two-tube kit is optimized to yield sensitive, unbiased representation over a broad dynamic range, with minimal setup and reaction time. Key Features and Benefits: Have ease of use and consistency — 2-tube (iScript reaction mix and iScript. Brendan. S from University of Arizona: Arizona Biomedical Collaborative reviews Bioline SensiFAST™ cDNA Synthesis Kit For more information about SensiFAST™ c.. GeneAce cDNA Synthesis Kitは、RNAを鋳型に1st strand cDNAを合成するためのキットです。逆転写酵素「GeneAce Reverse Transcriptase」を採用しているため、効率良く長鎖cDNAを合成することができます TruScript™ First Strand cDNA Synthesis Kit Product Insert Product # 54420 Norgen's TruScript™ First Strand cDNA Synthesis Kit is an all-in-one, ready-to-use product for simple set up of reverse transcription of total RNA (both poly A- or non-poly A-containing transcripts). The kit contains the 2x Reaction Mix and the TruScript™ Enzyme Mix. The 2x Reaction Mix contains a blend of buffer. ToolsQuant II Fast RT Kit is designed for the first strand cDNA synthesis with high efficiency and able to rapidly remove genomic DNA (gDNA). To avoid the contamination of gDNA, the kit contains gDNA Eraser that effectively remove gDNA (42°C, 3 min). It takes only 15 min (42°C) to complete the synthesis of first strand cDNA by using the innovative RT enzyme. This product also has a high.

Highly specific cDNA synthesis for qPCR - Qiage

SMARTer PCR cDNA Synthesis Kit Clontech (cat#s 634925 or 634926) KAPA™ HiFi PCR Kit Kapa Biosystems (PNs KK2101 or KK2102) Additional 5' PCR Primer IIA Any Oligo Synthesis Vendor 1.2% FlashGel® system or 0.80% Agarose Gels Lonza Any MLS FlashGel DNA Marker (100 bp - 4 kb) Lonza Qubit® dsDNA BR Assay Kit Life Technologies DNA 7500 Kit Agilent Template Prep Kit DNA/Polymerase Binding Kit. miRNA cDNA Synthesis Kit, with Poly(A) Polymerase Tailing. 产品描述: The miRNA cDNA Synthesis Kit is a complete system for the efficient synthesis of first strand miRNA from total RNA templates. The kit utilizes a mutated recombinant M-MuLV Reverse Transcriptase which exhibits limited RNase H activity. The recombinant RNasin™ Ribonuclease Inhibitor, supplied with the kit effectively. The FastSCRIPT™ cDNA Synthesis Kit contains everything you need to generate unbiased, efficient, sensitive and high-quality cDNA for your downstream experiments. Our kit removes the need for user optimization, making it an ideal solution due to its robust performance and ease of use. The kit contains a proprietary M-M cDNA oder ausgeschrieben complementary DNA ist eine Form der DNA, die mittels des Enzyms reverse Transkriptase aus RNA synthetisiert wird. 2 Synthese. cDNA kommt natürlicherweise nicht in menschlichen Zellen vor. Die Synthese erfolgt über eine PCR, bei der RNA, in der Regel mRNA, als Template eingesetzt wird. Je nach verwendetem Primer können einzelne Gene spezifisch oder die gesamte mRNA.

NZY M-MuLV First-Strand cDNA Synthesis Kit, separateSingle-cell transcriptome studies: a powerful way toPreamplification Supermix | Life Science Research | Bio-RadTissue Total RNA Isolation Kit (100 rxn) - FairBiotechComparative 454 pyrosequencing of transcripts from twoTaqMan Advanced miRNA Assays | Thermo Fisher Scientific - CN
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